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The Fastest Path To More Website Traffic

by Karla Taglioni @ Go Pro SEO | Michigan SEO Company

The Fastest Path To More Website Traffic Let’s say you have a local business and website. You’ll want to target the millions of Michigan consumers who are using the […]

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Interview Role Reversal – Episode 230

Interview Role Reversal – Episode 230

by David Johnston @ Photography Roundtable

You know, I’m very comfortable behind the microphone as the interviewer. I’m not so comfortable being the interviewee. However, my good friend Carl Van den Boom asked me to be on his new podcast, The Photopreneur Podcast, a while back. While I don’t always agree that I’m a good guest, I thought some of the ... Read more

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Hustle More – Episode 234

Hustle More – Episode 234

by David Johnston @ Photography Roundtable

The word “Hustle” gets thrown around a lot on the world wide web and it shouldn’t be. Why? Because the word “Hustle” is getting watered down. It’s losing its meaning. The severity of the word is becoming, well, less severe. We need to understand that if we want to improve as photographers we need to ... Read more

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Why Your Website Needs Professional SEO

by Karla Taglioni @ Go Pro SEO | Michigan SEO Company

Why Your Website Needs Professional SEO If you are reading this you’ve probably ran into many of the common frustrations that come along with attempting to […]

The post Why Your Website Needs Professional SEO appeared first on Go Pro SEO | Michigan SEO Company.

Transactivation of epidermal growth factor receptor through platelet-activating factor/receptor in ovarian cancer cells

Transactivation of epidermal growth factor receptor through platelet-activating factor/receptor in ovarian cancer cells


Journal of Experimental & Clinical Cancer Research

We previously identified platelet-activating factor receptor (PAFR) as being overexpressed in ovarian cancer and found that its ligand PAF evoked EGFR phosphorylation using the phospho-antibody microarray. Epidermal growth factor receptor (EGFR) are also overexpressed in ovarian cancer and contribute to the growth of ovarian cancer cells. Here, we investigated the mechanisms of crosstalk between PAFR and EGFR signaling in ovarian cancer cells to further determine whether the interaction between PAFR and EGFR synergistic contribute to the progression of ovarian cancer. Expression and localization of PAFR in several ovarian cancer cell lines were assessed by Western blot, realtime-PCR and immunofluorescence. The ovarian cancer cells were stimulated with PAF or PAF and in some experiments also pharmacological inhibitors. Phosphorylation of proteins in signaling pathways were measured by Western blot. HB-EGF concentrations of the supernatant from stimulated ovarian cancer cells were measured by enzyme-linked immunosorbent assay. Our data show that PAF increases EGFR phosphorylation through PAFR in a time- and dose- dependent manner in SKOV-3 ovarian cancer cells. This transactivation is dependent on phospholipase C-β and intracellular calcium signaling. This pathway is also Src tyrosine kinase- and metalloproteinase- dependent. PAF triggers EGFR activation through the increased heparin-binding EGF-like growth factor (HB-EGF) release in metalloprotease-dependent manner. Several studies involving EGFR transactivation through G-protein coupled receptor (GPCR) have demonstrated EGFR-dependent increase in ERK1/2 phosphorylation. Yet in SKOV-3 cells, PAF treatment also increases ERK1/2 phosphorylation in a EGFR-independent manner. The results suggest that in SKOV-3 ovarian cancer cells, PAF transactivates EGFR and downstream ERK pathways, thus diversifying the GPCR-mediated signal. The crosstalk between PAFR and EGFR suggests a potentially important signaling linkage between inflammatory and growth factor signaling in ovarian cancer cells.

Why Choose Our Local Michigan SEO Company?

by Karla Taglioni @ Go Pro SEO | Michigan SEO Company

Why Choose Our Local Michigan SEO Company? As a Troy based Michigan SEO Company, we want to help other Michigan businesses succeed. Our local SEO company has […]

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Real Estate Trends for 2017

by Marketing Team @ WolfNet

Tracking real estate trends is the realtor’s secret weapon for strategizing their sales process. Knowing what to expect gives you the advantage of communicating more clearly what your buyers and sellers can expect in the upcoming season. Setting realistic goals can also help you budget and forecast what your potential earnings could be by the […]

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Support Hurricane Relief Efforts with WolfNet

by Marketing Team @ WolfNet

Hurricane Harvey devastated Texas in late August.  The aftermath left 30,000 people displaced and at least $70 billion in damages. WolfNet is proud to be a part of the Keller Williams family which is headquartered out of Austin, Texas. As a leading provider of websites and responsive IDX, we have been serving KW agents and […]

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Get Higher Rankings With The Right SEO Formula

by Karla Taglioni @ Go Pro SEO | Michigan SEO Company

Get Higher Rankings With The Right SEO Formula Google looks for thousands of characteristics of a website before determining which sites will rank near the top […]

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We Guarantee 1st Page Results Within 90 Days

by Karla Taglioni @ Go Pro SEO | Michigan SEO Company

We Guarantee 1st Page Results Within 90 Days Most Michigan SEO companies, or any SEO Company for that matter, are unwilling to offer any type of […]

The post We Guarantee 1st Page Results Within 90 Days appeared first on Go Pro SEO | Michigan SEO Company.

Affordable Internet Marketing in Michigan

by Karla Taglioni @ Go Pro SEO | Michigan SEO Company

Affordable Internet Marketing in Michigan One of the most cost-effective ways to market your Michigan website is to have your site professionally marketed to the major […]

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A Trip Out West and More – Jon Secord Episode 231

A Trip Out West and More – Jon Secord Episode 231

by David Johnston @ Photography Roundtable

If my memory serves me correctly (which is a huge risk) I believe this is Jon Secord’s third time to come on the Photography Roundtable Podcast. I’ve had Jon on in the past to discuss his work and his process in planning locations, but I do enjoy the talks I get to have with good ... Read more

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DUI Lawyer and Drivers License Restoration Attorney in Flint, Grand Blanc, Clarkston, and Genesee County - Attorney Shaun Marks, Criminal DUI Drivers license lawyer | Flint | Clarkston | Michigan

DUI Lawyer and Drivers License Restoration Attorney in Flint, Grand Blanc, Clarkston, and Genesee County - Attorney Shaun Marks, Criminal DUI Drivers license lawyer | Flint | Clarkston | Michigan


Attorney Shaun Marks, Criminal DUI Drivers license lawyer | Flint | Clarkston | Michigan

DUI Lawyer Shaun Marks services Flint, Clarkston, Grand Blanc, Waterford and other areas helping people with their drunk driving cases.

Who Can Get My Website To The Top Of Google?

by Karla Taglioni @ Go Pro SEO | Michigan SEO Company

Who Can Get My Website To The Top Of Google? If you are wondering who can get my website to the top of Google, then the […]

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The Art of Winter Photography – Michael Bollino Episode 237

The Art of Winter Photography – Michael Bollino Episode 237

by David Johnston @ Photography Roundtable

About a year ago I invited Michael Bollino to be on the podcast to tell us about his photography and the process he shoots with. In this episode, Michael and I are going to be discussing the art of winter photography and what you need to know to shoot successful photographs in snow and icy ... Read more

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